A conserved core region of the scaffold NEMO is essential for signal-induced conformational change and liquid-liquid phase separation

Scaffold proteins help mediate interactions between protein partners, often to optimize intracellular signaling. Herein, we use comparative, biochemical, biophysical, molecular, and cellular approaches to investigate how the scaffold protein NEMO contributes to signaling in the NF-κB pathway. Comparison of NEMO and the related protein optineurin from a variety of evolutionarily distant organisms revealed that a central region of NEMO, called the Intervening Domain (IVD), is conserved between NEMO and optineurin. Previous studies have shown that this central core region of the IVD is required for cytokine-induced activation of IκB kinase (IKK). We show that the analogous region of optineurin can functionally replace the core region of the NEMO IVD. We also show that an intact IVD is required for the formation of disulfide-bonded dimers of NEMO. Moreover, inactivating mutations in this core region abrogate the ability of NEMO to form ubiquitin-induced liquid-liquid phase separation droplets in vitro and signal-induced puncta in vivo. Thermal and chemical denaturation studies of truncated NEMO variants indicate that the IVD, while not intrinsically destabilizing, can reduce the stability of surrounding regions of NEMO due to the conflicting structural demands imparted on this region by flanking upstream and downstream domains. This conformational strain in the IVD mediates allosteric communication between the N- and C-terminal regions of NEMO. Overall, these results support a model in which the IVD of NEMO participates in signal-induced activation of the IKK/NF-κB pathway by acting as a mediator of conformational changes in NEMO.

Intracellular processes require the precise coordination of molecular components for optimal output.In many cases, scaffold proteins facilitate these intracellular processes by acting as docking hubs for interacting partners in a given signaling pathway (1).However, scaffold proteins are not simply inert frameworks for such coordinated events.That is, scaffold proteins can themselves contribute to these multicomponent molecular processes by undergoing controlled conformational changes that bring multiple components into the proper configuration for downstream signaling.
One such multicomponent process is the transcription factor NF-κB pathway, which regulates myriad cellular and organismal processes including immunity, development, and cell survival (2).In most resting cells, NF-κB dimers are sequestered in the cytoplasm in a complex with inhibitor of NF-κB (IκB) proteins, which are substrates for regulated phosphorylation by the IκB kinase (IKK).In canonical NF-κB signaling, activated IKKβ carries out the phosphorylation of IκB (primarily IκBα), which is then followed by degradation of IκB, and the liberation of NF-κB for translocation to the nucleus and activation of NF-κB target genes (3,4).The canonical IKK complex is composed of the kinases IKKα and IKKβ and the scaffold NEMO (also called IKKγ) (3).Knockouts and mutations of NEMO abolish the ability of cells to respond to canonical NF-κB pathway activators, such as tumor necrosis factor alpha (TNFα) and interferon beta (IFNβ) (5,6).Thus, NEMO is an essential scaffold for the IKKβ-dependent phosphorylation of IκBα, but the mechanism by which NEMO exerts its scaffolding activity is not completely understood.
Human NEMO is a 419 amino acid protein with several subdomains that contribute to its scaffolding function and hence NF-κB signaling.As shown in Figure 1A, these domains include a primary N-terminal binding domain for IκB kinase beta (IKKβ) (KBD, aa 44-111), a ubiquitin-binding domain (UBAN, aa 286-321), and a C-terminal zinc finger domain (ZF, aa 389-419) (7)(8)(9)(10).One region of NEMO (aa 110-195, called the Intervening Domain [IVD]) is not known to bind any other proteins/ligands, and its precise function is not known.Nevertheless, some mutations in conserved core sequences of the IVD abolish the ability of NEMO to support cytokine-induced activation of IKK (5,11).Although the overall structure of NEMO is likely to be a series of coiled-coil domains, the X-ray crystallographic structure of the IVD is yet to be determined.
Mutations in NEMO that reduce or abolish its ability to support downstream activation of NF-κB are the cause of several human immunodeficiency diseases (5).In many cases, these mutations interfere with the ability of NEMO to interact with its key protein partners (8,9,21,22).Nevertheless, several human disease mutations located in the IVD are not known to affect NEMO-dependent protein-protein interactions (see Fig. S1), although several reduce the ability of NEMO to support cytokine-induced activation of NF-κB (5).Moreover, we have previously shown that substitution of a highly conserved region of the IVD (aa 145-153, replaced by a Ser-Gly repeat; mutant 9SG) abolishes the ability of NEMO to support TNFα-, LPS-and IL-1β-induced phosphorylation of IκBα, although this mutation does not block the interaction of NEMO with IKKβ (11,13).Furthermore, two human disease mutations within this core sequence (L153R, V146G) fail to support activation of NF-κB in response to a variety of ligands, including TNFα, Toll-like receptor agonists, CD40, anti-CD3, and anti-CD28 (6,23,24).
In this study, we have used comparative, molecular, biochemical, biophysical, and cellular approaches to understand the role of the IVD in conformational changes in NEMO that are required for the activation of IKK.Our results support a model in which the IVD is a sequence-dependent driver of the conformational changes and LLPS that are required for Figure 1.Evolutionary analysis and comparison of NEMO and OPTN proteins.A, NEMO domain structure, with colored areas corresponding to the functional domains of NEMO and proposed unstructured regions represented by black lines.HLX1/KBD, Helix 1/Kinase Binding Domain; CC1/IVD, Coiled-Coil 1/Intervening Domain; HLX2, Helix 2; CC2, Coiled-Coil 2; LZ, Leucine Zipper; UBAN, Ubiquitin Binding in ABIN and NEMO domain; ZF, Zinc Finger.B, plot of the sequence conservation obtained using Rate4Site and the residue hydropathy calculated using the Kyte & Doolittle scale for human NEMO, represented by a moving average.The red bars denote the location of the hot spot regions of the NEMO-IKKβ binding interaction (Fig. S2).C, structural comparison of NEMO and OPTN protein domain structures, with the black dotted line indicating a gap in the alignment.LIR, LC3 Interacting Region.D, multiple sequence alignment for the IVD core region of NEMO and OPTN, from a broad spread of vertebrate proteins.Sequences were aligned with Clustal Omega (EMBL-EBI) using WT human NEMO as a reference sequence.The sequence alignment was visualized and colored according to residue conservation using MView (EMBL-EBI) (60).
activation of IKK/NF-κB.As such, these findings suggest that mutations in the IVD affect the ability of NEMO to undergo stimulus-induced conformational change and can be classified as a new type of scaffolding mutation leading to disease.Our model provides insights for therapeutic design for disease treatment.

Results
Comparative sequence analyses of the IVD region of NEMO suggest functional importance Shaffer et al. (13) highlighted the highly conserved core region of the IVD as critical for NEMO function and proposed a role for IVD-mediated conformational change in the downstream signal propagation of the NF-κB pathway.To provide additional insight into the structure/function relationship in the IVD, we determined how the evolutionary rate of the IVD compared to other structurally characterized domains of NEMO that participate in protein-protein interactions.We used the tool Rate4Site (25) to construct a phylogenetic tree from a multiple-sequence alignment, using the neighbor-joining algorithm, which estimates the maximum likelihood evolutionary rate for each residue position.The rates are normalized around zero with a standard deviation of one, with positive scores representing higher and negative scores representing lower rates of evolution.We used 500 NEMO sequences from vertebrate species (sequence identity of 64-100%) to perform this analysis using a four-period moving average to better uncover trends of functional importance in NEMO (Fig. 1B).The analysis shows a region of low evolutionary rate that coincides with the 44 to 111 KBD region of NEMO.The affinity of the partners in this proteinprotein binding interface is dependent on three "hot spots" which were experimentally validated and independently identified by computational structural mapping (26).These hot spots align well with the sites of the lowest evolutionary rate in the KBD region (Fig. S2).In addition, the HLX-2 (Helix 2) domain, the CC2 domain, and the UBAN region that spans the CC2 and LZ domains, which contain defined segments that participate in protein-protein interactions (27), all show low rates of evolutionary change in the expected regions.Therefore, the low evolutionary rate of the IVD (Fig. 1B) is similar to regions of NEMO that are known to be of functional importance.The evolutionary rate within the IVD is lowest for residues 141 to 161, suggesting a critical role for this core region.In contrast, the N-terminal disordered region of NEMO comprising residues 1 to 44 and the C-terminal disordered region (residues 345-388) have high rates of evolutionary divergence, suggesting they do not have functional roles that require sequence conservation.
As a second method of identifying key regions of NEMO, we performed a hydrophobicity analysis, using the Kyte & Doolittle scale to score the hydrophobicity of each residue subjected to a moving average.NEMO is primarily an extended coiled-coil protein with high solvent exposure and thus displays a low average hydrophobicity.Therefore, especially hydrophobic regions are likely either to take part in protein-protein interactions or to comprise folded domains large enough to have a hydrophobic core.Indeed, the proteininteracting regions within the HLX-2, UBAN, and CC2 domains all display spikes of increased local hydrophobicity (Fig. 1B).Thus, there is a general correlation between low evolutionary rate and high hydrophobicity in these domains with known functional roles.Similarly, for the IVD, an area of high hydrophobicity overlaps with the area of low evolutionary rate, suggesting that the IVD is involved in protein-protein interactions and/or forms a compact folded domain (Fig. 1B).
The evolutionary rate analysis can be further used to score the order/disorder of a region and its propensity for forming protein-protein interactions by computing evolutionary ratios.Dubreuil et al. (28) determined that the average evolutionary rate of a disordered region divided by the average evolutionary rate of residues in the ordered domains of proteins from a large dataset of proteins of known structure yields a ratio of 2. For NEMO, we find ratios greater than three for both the N-terminal and C-terminal disordered regions, as expected, but a ratio of 1.4 for the central core of the IVD.The use of this ratio as a metric suggests that the IVD is not a fully disordered region, but it is not as ordered as other known structured domains of NEMO such as the HLX-2, CC2, and LZ (with ratios of 0.52, 0.81, and 1.1, respectively).
To identify possible homologs of NEMO, we generated a sequence similarity network (SSN) (Fig. S3) using human NEMO as a query.All proteins in the NEMO SSN cluster had 40 to 99% identity to human NEMO and appeared to be NEMO orthologs.A group of proteins with 22 to 31% sequence identity with human NEMO formed a second isofunctional cluster and were uniquely annotated as optineurin (OPTN), a protein whose sequence similarity to NEMO has been recognized previously (29).
Alignment of the complete sequences of 38 vertebrate NEMO and OPTN proteins (Fig. S4) showed that the domain organization of OPTN and NEMO are similar except for the presence of additional central LZ and LIR domains in OPTN (Fig. 1C).In particular, both NEMO and OPTN have extensive sequence similarity in the core IVD sequence (Fig. 1D).

Substitution of the core sequence of the NEMO IVD with analogous sequences from OPTN does not affect the ability of NEMO to support TNFα-induced activation of IKKβ
Based on the sequence conservation of the core region of the IVD in both NEMO and OPTN proteins, we sought to determine whether these core regions were functionally similar.To do this, we created relevant mutations in the core region of NEMO, subcloned them into FLAG-tagged expression plasmids (Fig. 2A), and transfected the plasmids into 293T NEMO knockout cells (11).To assess the function of NEMO mutants, transfected cells were treated with TNFα, and TNFαinduced phosphorylation of IκBα was compared to that seen in empty vector-transfected cells (negative control) and to wildtype NEMO-transfected cells (positive control).
We first tested whether the replacement of the most conserved region of NEMO's IVD (aa 142-159) with the analogous region of OPTN (aa 302-319) affected NEMO's signaling function in vivo.This segment of OPTN contains nine aa differences from the analogous region in NEMO, of which seven are conservative changes (V142L, K143N, L150F, S156A, Q157H, S158T, R159K) and two are not conservative (A144L, G151K) (Fig. 2A).The substitution mutant OPTN-IVD-NEMO showed no significant difference from wild-type NEMO in supporting TNFα-induced phosphorylation of IκBα (Fig. 2, B and C).Thus, the conserved core IVD sequences of OPTN can functionally substitute for the core region of NEMO.In contrast, a NEMO mutant with a deletion of aa 145 to 153 (Δ145-153) showed a significant reduction in TNFα-induced phosphorylation of IκBα.
Since L153 is one of the most conserved residues (by sequence alignment, Fig. 1D) and the L153R mutation is detrimental to NEMO function (6,11), we also tested if adding back only L153 would restore function to the inactive 9SG NEMO mutant, which has a repeating Ser-Gly replacement from aa 145 to 153 (11,13) (Fig. 2A).This new mutant (9SG-L153) showed no significant ability to support the phosphorylation of IκBα, confirming that the functional importance of the highly conserved core region of NEMO does not rely simply on L153.Taken together, these results confirm our comparative analysis showing that the core region of the IVD is important for NEMO function and that the analogous region of OPTN can functionally replace this core region of NEMO even though their sequences are not precisely conserved.
We also determined whether replacement of the core region of NEMO's IVD with the corresponding segment of OPTN affected the ability of NEMO to bind IKKβ in vitro, using our previously established fluorescence anisotropy binding assay that uses an FITC-labeled 45-mer peptide derived from the C-terminal region of IKKβ (13,26).For these assays, as well as the CD measurements described below, we used a form of NEMO in which seven Cys residues have been substituted with Ala (7XAla-NEMO, Fig. S5) because we have previously shown that this variant of NEMO is less susceptible to disulfidebonded aggregate formation when expressed in bacteria and yet retains WT NEMO activity in human cells (30,31).Fulllength NEMO proteins (aa 1-419), including WT NEMO, 7XAla-NEMO, and 9SG-NEMO, bind the 45-mer peptide derived from IKKβ with affinities ranging from 2 to 10 nM (26,31).Using the same method, we found that OPTN-IVD-NEMO bound to the 45-mer peptide with K D 33 ± 14 nM, which is slightly higher than the values seen for WT (4.7 ± 0.64 nM) or 7XAla -NEMO (13 ± 2.9 nM) (Figs.2F and S6).Nevertheless, all three NEMO proteins (WT, 7XAla-NEMO, and OPTN-IVD-NEMO) fully support TNFα-induced activation of IKKβ in vivo.
To compare the structures of NEMO and OPTN-IVD-NEMO, we performed circular dichroism (CD) on bacterially expressed NEMO proteins in the presence and absence of bound IKKβ peptide (Fig. 2D).By CD, full-length WT NEMO and 7XAla-NEMO displayed nearly identical secondary structures strongly dominated by alpha helices, with a ratio of ellipticity at 222/208 nm of 1.02 and 1.00, respectively, indicating a high coiled-coil content.For OPTN-IVD-NEMO, we observed a similar α-helical structure with a decrease in the ellipticity ratio to 0.91, suggesting a small reduction in the coiled-coil content following the insertion of the IVD from OPTN into NEMO.We also compared the structural stability of the NEMO variants by determining their melting temperatures.Melting was measured by monitoring the change in the 222 nm peak in the CD spectrum as the temperature was ramped from 10 to 70 C. OPTN-IVD NEMO was found to have a melting temperature of T m = 42 C± 0.54 deg.C, while 7XAla and WT NEMO had T m values of 39 C ± 0.78 deg.C and 40 C ± 0.71 deg.C, respectively (Fig. 2E) Thus, the OPTN-IVD substitution did not reduce the stability of the fulllength NEMO protein.Moreover, the inclusion of an equimolar concentration of the IKKβ 45-mer peptide caused an increase in T m of 3 C for WT NEMO and 7XAla-NEMO and of 5 C for OPTN-IVD-NEMO.Overall, these results indicate that substitution of the OPTN-IVD in NEMO does not substantially alter full-length NEMO's secondary structure, stability, or interaction with IKKβ.

Disruption of the IVD impairs disulfide-bonded dimer formation of NEMO in vivo
Although the NEMO dimer is natively noncovalent in the reducing environment of the cell (31), certain cysteine residues, such as Cys54, are positioned at the dimer interface such that interchain disulfide bonding can occur if the protein is subjected to oxidizing conditions (32).We have previously shown that NEMO can form disulfide-bonded dimers in cells either when lysates are made in the absence of a reducing agent or following the treatment of cells with hydrogen peroxide (30,31).To determine whether IVD mutations affect disulfide-bonded dimerization of NEMO, we first expressed FLAG-tagged versions of full-length WT, 9SG, and OPTN-IVD-NEMO proteins in 293T NEMO knockout cells.Cells were then lysed with a non-ionic detergent buffer and the lysates were analyzed by non-reducing (i.e., no β-mercaptoethanol or boiling) SDS-PAGE.We observed that WT NEMO weight markers (in kDa) are indicated to the left.The dotted line separates two sections of the same blot that were probed with the antibody indicated on the right.C, quantitation of IκBα phosphorylation from at least three experiments per transfection condition.The amount of phosphorylation of IκBα is expressed as the ratio of phospho-IκBα to NEMO relative to the average ratio of wild-type NEMO transfected cells (100).Shown are the means ± SEM; ns, not significant; *p < 0.05; **p < 0.01 by t test assuming equal or unequal variance as appropriate; n = ≥ three individual experiments.Dots indicate the values of individual experiments.D, The CD spectra of indicated NEMO constructs determined at 25 C at 10 μM, with and without equimolar equivalents of a 45-mer peptide of IKKβ, incubated for 1 h at room temperature.E, CD-monitored thermal denaturation of NEMO constructs by measuring the loss in secondary structure as an increase in the 222 nm signal, ± equimolar equivalents of a 45-mer peptide of IKKβ, incubated for 1 h at room temperature.Insets show the peak of the first derivative as the melting temperature (T m ).F, table of key CD results.The ellipticity ratios of the constructs is indicated as the ratio of the signal at 222 nm to 208 nm with and without the IKKβ peptide in (B), T m with and without IKKβ peptide in (D), and K D values for the binding affinity of the constructs for a FITC-labeled 45-mer peptide of IKKβ determined using a fluorescence anisotropy assay (and see Fig. S6).migrated as two bands, with one band at the position expected for a NEMO monomer and a second higher band at approximately double the molecular weight, that is, corresponding to dimeric NEMO.The active OPTN-IVD NEMO mutant also showed this two-band pattern and had a dimer/monomer ratio that was similar to that seen with WT NEMO.In contrast, no higher molecular-weight band was seen with 9SG NEMO (Fig. 3A), although there are no Cys residue substitution mutations in 9SG.On fully denaturing SDS-PAGE bacterially expressed versions of these three proteins co-migrated as single bands (see below, Fig. 4D).
As a second measure of oxidative dimer formation, we assessed the ability of the NEMO 9SG mutant to form disulfide-bonded dimers in cells following hydrogen peroxide treatment.We have previously shown this disulfide-bonded dimerization of NEMO involves Cys54 and Cys 347 (30).Therefore, we transfected 293T NEMO KO cells with FLAGtagged versions of full-length WT, 9SG (WT background), and C54/347A NEMO proteins, treated the cells with hydrogen peroxide, and then subjected the lysates to nonreducing (but with boiling) SDS-PAGE (30).Under these conditions, abundant dimer formation was seen for WT NEMO, but not for the NEMO mutants Cys54/347Ala or 9SG (Fig. 3B).Given that Cys54 and Cys347 are intact in the 9SG mutant, its inability to form disulfide-linked dimers in the presence of hydrogen peroxide suggests that 9SG-NEMO has an altered conformation such that Cys54 and 347 are no longer suitably positioned within the noncovalent NEMO homodimer to form disulfide bonds.
Overall, the results in this section show that the 9SG mutation alters the reactivity of cysteine residues in other regions of the protein, indicating that mutational disruption of the IVD alters the structure of the NEMO dimer in a way that propagates to distant parts of the protein.
An inactivating mutation of IVD residues 145 to 153 perturbs ubiquitin-induced liquid-liquid phase separation of NEMO Three studies (18)(19)(20) have recently shown that ubiquitin can stimulate the LLPS transition of purified NEMO in vitro and that mutations that remove the C-terminal ubiquitinbinding domain of NEMO abolish this induced transition.Given our results above indicating that perturbation of the IVD interfered with the ability of NEMO to form higher order structures, we next determined whether IVD mutation affected LLPS of NEMO.Specifically, we compared LLPS formation of full-length and 9SG NEMO in the presence of M1-octa-Ubiquitin (M1-Ub 8 ) (18,19).For the LLPS assay, we first purified full-length WT NEMO, 7XAla-NEMO, and 9SG-NEMO (7XAla background) expressed in E. coli and labeled the purified proteins with Alexa Fluor 488.The Alexa Fluorlabeled NEMO proteins were then mixed with increasing concentrations of M1-octa-Ubiquitin (M1-Ub 8 ), and the reaction mixtures were monitored for liquid droplet formation by fluorescence microscopy.We found that both WT NEMO and 7XAla NEMO readily formed droplets in the presence of M1-Ub 8 , but few droplets were seen with 9SG-NEMO (Fig. 4A).The total raw fluorescence for both WT and 7XAla NEMO increased with increasing concentrations of M1-Ub 8 , whereas the total raw fluorescence of 9SG-NEMO did not increase under these conditions (Fig. 4B).In addition, at the same sub-saturating concentration of M1-Ub 8 , 7XAla showed approximately 2.5-fold more LLPS compared to WT NEMO.In contrast, 9SG-NEMO showed only approximately 10% of the total fluorescence of its positive control 7XAla-NEMO (Fig. 4C).The bacterially expressed proteins used in the LLPS  assays are shown in Figure 4D.Overall, these results demonstrate that the 9SG IVD mutation interferes with ubiquitininduced LLPS of NEMO.

NEMO and conformational change
Since the 9SG mutation is not within the ubiquitin-binding region of NEMO, we sought to confirm that the effect of this IVD substitution on LLPS was not due to a failure of the protein to interact with ubiquitin.For these experiments, we incubated bacterially expressed GST alone or GST-diubiquitin (Ub 2 ) with cell lysates from 293T NEMO KO cells overexpressing FLAG-tagged NEMO proteins and performed a pulldown assay as described previously (33).We used cell lysates expressing FLAG-tagged versions of WT, 7XAla, 9SG, or L153R NEMO, all of which have intact C-terminal Ub-binding domains.All four NEMO proteins were pulled down by GST-Ub 2 but not by GST alone (Fig. 4E).These results indicate that the inability of the 9SG NEMO mutant to undergo LLPS is not due to an impaired ability to bind to ubiquitin.
Taken together, the results in this section demonstrate that the core IVD sequence of NEMO is required for the LLPS that is induced by binding to ubiquitin in vitro.

Mutation of IVD residues 145 to 153 perturbs the formation of induced puncta of NEMO in vivo
Previous studies have shown that induced activation of the NF-κB pathway in vivo can lead to the formation of higher order complexes containing NEMO, which can be observed as cytoplasmic puncta (34,35).Furthermore, infection with mycoplasma has been shown to lead to constitutive activation of NF-κB in the chicken fibroblast cell line DF-1, due to chronic activation of upstream TLR6 (36).For our studies, we also chose to study induced puncta formation of NEMO proteins in DF-1 cells, in part because this cell line has an easily discernible "spread out" morphology.
We first confirmed that mycoplasma infection of DF-1 cells leads to constitutive activation of NF-κB by showing that infection of DF-1 cells with mycoplasma increased nuclear translocation of NF-κB p65 as compared to uninfected DF-1 cells (Fig. S7).We next used a surrogate for mycoplasma infection by treating cells with a synthetic lipoprotein mimetic, Pam2CSK4, which has been shown to cause NF-κB activation in other cell lines (37).As judged by nuclear translocation of NF-κB p65, Pam2CSK4 caused increased NF-κB activation following 4 h of treatment (Fig. 5A).We therefore used the 4 h time point for the activation of NF-κB in our subsequent experiments.
To determine whether IVD perturbations affect NEMO puncta formation in DF-1 cells, we transfected DF-1 chicken fibroblasts with FLAG-tagged NEMO, 9SG NEMO, or the human disease L153R mutant of NEMO.Two days later, transfected cells were treated with Pam2CSK4 for 4 h.We then performed anti-FLAG indirect immunofluorescence (IF) and counted the number of large cellular puncta (≥1 μm).Cells transfected with WT NEMO and treated with Pam2CSK4 had significantly higher numbers of puncta as compared to the corresponding untreated control cells, indicative of higher order complex formation, as consistent with previously published results (Fig. 5, B and C) (34,35).In contrast, cells transfected with the inactive 9SG and L153R NEMO mutants did not show increased numbers of puncta per cell following treatment with Pam2CSK4.Similar results were obtained by infection of cells with mycoplasma (Fig. S7).These results indicate that NEMO proteins with inactivating IVD mutations have an impaired ability to form higher order puncta in vivo following treatment with an activator of NF-κB and are consistent with the findings above that an inactivating IVD mutation reduces the ability of NEMO to undergo LLPS in vitro.
We have previously shown that FLAG-NEMO, as well as various active NEMO variants, can interact with His-tagged TRAF6 in co-immunoprecipitation assays from cotransfected 293T cells (32).Therefore, we also used this assay to investigate whether the IVD mutations 9SG and L153R affected the interaction of NEMO with the TNF-R signaling protein TRAF6.As shown in Figure 5D, WT NEMO and 9SG NEMO pulled down TRAF6 to a similar extent, but the L153R mutant had a substantially reduced ability to pull down TRAF6.

Impact of the IVD on NEMO structure and stability
Previous work has suggested that the IVD acts to destabilize the coiled-coil structure of NEMO (15).To gain additional insight into the role of the IVD, and NEMO's other domains, on the stability of the protein we generated a set of NEMO constructs with varying degrees of truncation N-terminally and/or C-terminally to the IVD (Fig. 6A).We tested the effect of these truncations on the thermostability of the protein using variable-temperature CD (Figs. 6, B and D and S8).As described earlier, the T m of full-length WT NEMO (1-419) was 40 C ± 0.71 deg.C, which is consistent with previously published results (13).As expected, removal of the N-terminal disordered region from residues 1 to 43 (i.e., NEMO 44-419) had minimal effect on T m (41 C ± 0.23 deg.C), likely because this N-terminal region is largely unstructured (38).
Of note, the constructs NEMO 196 to 419 and NEMO 252 to 419, which include only domains C-terminal to the IVD (and not the IVD itself), are considerably more stable (by 20 C) than WT NEMO, having melting temperatures of T m = 60 C ± 1.1 deg.C for NEMO 252 to 419 and T m = 60 C ± 0.75 deg.C for NEMO 196 to 419.Thus, the presence of portions of NEMO N-terminal to residue 196 substantially destabilizes the protein in the presence of thermal denaturation, consistent with the notion that the IVD and/or the KBD exert a destabilizing effect on the HLX2 and the CC2/LZ domains which both comprise α-helical coiled-coils (32,39).
Peptides comprising only the core conserved region of the IVD (NEMO 141-155), or even the full IVD (NEMO 110-195), do not show cooperative thermal unfolding events.Indeed, these small fragments are likely monomeric and disordered given that they lack the flanking domains that are mainly responsible for NEMO dimer stabilization (15).6, B and D).This result again shows that the KBD together with the IVD serves to destabilize "downstream" domains.Thermal denaturation results from nanoDSF corroborated these findings for select constructs (Fig. 6D).
Thermal denaturation of proteins is often irreversible, and thus a T m value typically cannot be interpreted as a measure of thermodynamic stability as it is affected by kinetic factors arising from conditions such as protein concentration and  model allows extrapolation to determine the Gibbs free energy of unfolding at zero urea (40,41).The results of these experiments largely mirrored those from the thermal denaturation measurements.Specifically, the two constructs containing only those domains downstream of the IVD, that is, NEMO 196 to 419 and NEMO 252 to 419, showed high free energies of unfolding of ΔG u = 3.2 to 3.3 ± 0.2 to 0.4 kcal/mol, whereas NEMO 44 to 419, which additionally contains the KBD/IVD region, showed substantially reduced thermodynamic stability with ΔG u = 2.2 ± 0.2 kcal/mol.Thus, the KBD and IVD collectively destabilize the NEMO dimer to the extent of 1.1 kcal/mol relative to NEMO constructs that contain only the HLX2 and CC2/LZ domains.
We additionally examined the CD spectra to assess the percent α-helix of the same constructs, and found a similar trend of the IVD decreasing the percent α-helix of the adjacent domains.When we calculated the percent α-helix from our CD spectra (Fig. 6D), we found that NEMO 44 to 419 and 44 to 195 have high α-helical content as we have previously shown (66% and 69%, respectively) (13).For the conserved core of the IVD (aa 141-155) alone, we measured a percent αhelix of 28%, which increases to 35% for the full IVD (110-195), and to 69% upon the addition of the KBD and IVD domains (aa .The C terminus of NEMO (aa 344-419) has an extremely low percent α-helical content (9%) that increases as the LZ (310-419), CC2 (aa 252-419), and HLX-2 (aa 196-419) are added (52%, 65%, and 64%, respectively).When the IVD is added between the KBD and HLX-2 domains, a decrease in percent α-helix (to 60%) is observed, indicating a disruption in the secondary structure.These results are consistent with those from our T m measurements and indicate that the IVD affects the conformation of adjacent domains.
To determine how much of this destabilization effect can be attributed to the IVD versus the KBD, we subjected 1 to 419 9SG (in the 7XAla NEMO background) to similar analyses.The CD spectrum of full-length 9SG (Fig. 6E) is similar to that of 7XAla (Fig. 2D), in agreement with our previous findings (13), showing that the loss of biological function seen for the 9SG mutant is not due to a major disruption of NEMO's overall secondary structure.In the urea denaturation experiments, the free energy of unfolding for the 1 to 419 9SG mutant was ΔG u = 2.8 ± 0.2 kcal/mol, compared to 2.2 ± 0.1 kcal/mol for the 7XAla control (Fig. 6, F and G).These results show that disruption of the core region of the IVD by introducing the 9SG substitutions mitigated the stability difference caused by the KBD/IVD regions of NEMO, suggesting that this effect is largely attributable to destabilizing interactions involving the IVD.
In contrast to what is seen with the 9SG substitution, thermal denaturation of the full-length (aa 1-419) NEMO L153R mutant gave a melting temperature of T m = 32 C ± 1 deg.C, which is approximately 8 C lower than WT NEMO, and opposite to the stabilization of NEMO that was seen for the 9SG substitution (Fig. S10).A previous publication reported that the L153R substitution increased T m (15), however, that conclusion was based on a rapid temperature ramp method which can give unreliable results (42).Our thermal denaturation results indicate that while L153R and 9SG both abolish NEMO activity, they do so by different mechanisms.
We also measured the effects of binding the IKKβ 45-mer peptide on the secondary structure and stability of different forms of NEMO.The addition of equimolar equivalents of the IKKβ peptide to WT NEMO, 7XAla-NEMO, or OPTN-IVD-NEMO in each case resulted in a substantial (3-5 C) increase in thermal stability, as might be expected for the addition of a high-affinity ligand (43).No change in the coiled-coil content was observed upon IKKβ peptide binding to any of these three NEMO variants, consistent with the notion that the KBD region (aa 44-111) is preorganized as a coiled-coil even in the unbound state.In contrast, when 1 to 419 9SG was incubated with the IKKβ peptide, no T m increase was observed, but there was a large increase in the ellipticity ratio from 1.02 to 1.44, indicating an increase in coiled-coil structure (Fig. 6H).This result suggests that the KBD region is disrupted in the unbound 9SG mutant, presumably because the disrupted IVD structure fails to correctly organize dimerization of the KBD, as has been shown to be necessary for proper coiled-coil formation (40,44).Consequently, the binding energy that results from interaction with the IKKβ peptide is utilized to induce coiled-coil formation, with essentially none remaining to stabilize the protein.This conclusion is consistent with the differences between these NEMO constructs observed in prior small angle X-ray scattering analyses, which showed an overall lengthening of 9SG NEMO upon the addition of the IKKβ peptide, in contrast to the compaction seen when WT NEMO bound the peptide (13).

Discussion
The data presented herein further our understanding of the role of a conserved central region of NEMO (especially residues 145-153 of the IVD) that is required for the full activity of NEMO in downstream activation of IKKβ and ultimately the activation of NF-κB.We show the extent of conservation of this region in NEMO proteins across a broad evolutionary swath, as well as sequence and functional conservation of the corresponding sequence from the NEMO-related protein optineurin (OPTN).Namely, we show that replacement of NEMO IVD sequences with the analogous OPTN sequences does not alter NEMO structure or function, whereas removal or replacement of aa 145 to 153 with a more flexible sequence (i.e., the Δ145-153 and 9SG mutants, respectively) rendered NEMO nonfunctional.The core IVD sequences are also required for LLPS in vitro and signal-induced puncta formation in vivo.Finally, we have used biochemical and biophysical methods to characterize the effects that the IVD region has on NEMO structure, stability, conformational change, and higher order structure formation upon the binding of ligands, which lead to a model for how these attributes contribute to NEMO functionality.Overall, these results add to the mounting evidence that, during the course of NEMO's scaffold signaling function, NEMO undergoes several conformational rearrangements upon binding various ligands, including IKKβ, IκBα, and poly-ubiquitin (12, 13, 15-17).
We previously suggested that the IVD was a mediator of conformational changes by showing that compaction of NEMO occurs upon the binding of a 45-mer peptide derived from IKKβ and that mutation of residues 145 to 153 abolishes this effect (13).However, the lack of an experimental X-ray crystal structure or convincing structural model of the IVD left many questions unanswered about its architecture and function.Recent reports that NEMO engages in LLPS and that certain human disease mutants in NEMO that lack the ability to bind to linear ubiquitin are defective for LLPS in vitro, for the formation of higher order complexes in vivo, and for supporting induced NF-κB signaling (18,19) add additional layers of complexity to NEMO's mechanism of action.
How different conformational states are molecularly advantageous for NEMO function is not entirely known.One emerging model is that upon TNFα-induced activation of the NF-κB pathway, the NEMO-IKKβ complex is brought to the TNFR receptosome by binding to ubiquitin chains on the adaptor protein RIP.Further modification of NEMO with ubiquitin via LUBAC alters the conformational state of the NEMO complex, which is likely modulated, in part, by the IVD.This change in the conformational state then provides a switch required for higher order IKK signalosome formation and LLPS of IKK signaling components, namely, NEMO, IKKβ, IκBα, TRAF, TAK/TAB, and LUBAC.Based on our biophysical data, we hypothesize that the IVD regulates the structure of NEMO by acting as a central hub to reorganize the flanking coiled-coil domains.This model is also consistent with observations by Ko et al. (15) who demonstrated that mutants lacking or containing mutations in the IVD exhibit altered solution dynamics, indicating perturbation in the ability of NEMO to oligomerize.
Our demonstration that disruption of the core sequences in the IVD (mutant 9SG) abolishes the ability of NEMO to undergo ubiquitin-induced LLPS coincides with the failure of this mutant to support activation of IKK in response to TNFα (11,13).Moreover, we show that neither the 9SG mutant nor the L153R immunodeficiency disease mutant, which is also defective for IKKβ activation (11), form high molecular weight puncta in response to a TLR agonist.Thus, internal (e.g., IVD) mutations in NEMO also appear to disable NEMO signaling to IKK, by affecting LLPS and higher order signalosome formation, although such mutants can still bind to ubiquitin (Fig. 4E).Overall, we suggest that the 9SG and L153R mutations cripple NEMO function by disabling its ability to undergo a conformational change that is required for LLPS.Whether other NEMO human disease mutants-in the IVD or elsewhere-also affect LLPS is not clear.Our data are also consistent with puncta forming in stimulated cells via LLPS (18) in that an intact IVD core sequence is crucial for both LLPS of NEMO and for puncta formation in cells.Specifically, the 9SG mutation reduces LLPS, and the 9SG and L153R mutants do not show increased signal-induced puncta formation in cells.
Considering what we know about the conformational flux of NEMO upon ubiquitin binding and the solvent exposure of residues distal to the ubiquitin-binding region (16), it is possible that LLPS of NEMO complexes is driven by the exposure of key residues that are buried residues in the resting state.A similar finding has been made for the scaffold HP1α that undergoes LLPS with heterochromatin.HP1α also has a low complexity hinge region that is important for LLPS, which is driven by ion concentrations that cause conformational changes in HP1α.It has been proposed that this reorganization of HP1α leads to solvent exposure of hydrophobic/charged residues and enhances self-oligomerization, which then promotes LLPS (45).It is presently unclear whether the deficiency in IκBα phosphorylation that is seen with the 9SG and L153R mutants (11,13) is a result of these defects in LLPS or instead represents a different consequence of the disruption of IVD structure and function.
Our biophysical results also shed new light on the molecular details of how the IVD performs its critical functions.The results of the thermal and chemical denaturation experiments establish that the IVD mediates an intimate allosteric communication between the flanking KBD and the HLX2 and CC2/LZ domains.Evidence that the IVD is allosterically coupled to the HLX2 and CC2/LZ domains is provided by the observation that the presence of the KBD and IVD substantially destabilizes the protein compared to constructs in which these domains are deleted, namely NEMO 195 to 419 and NEMO 252 to 419, and this destabilization is largely relieved when the structure of the IVD is modified by replacement of its highly conserved core region with a flexible linker in the 9SG mutant.These findings could be taken as evidence that the homodimer interface in the IVD region is intrinsically destabilizing.However, our results indicate that this is not the case.Inclusion of the IVD induces the otherwise largely disordered KBD region to adopt an ordered structure with substantial thermal stability, as shown by the thermal and chemical denaturation results for the KBD-IVD construct NEMO 44 to 195 as compared to the behavior of constructs containing only the KBD (7,32,38).This result shows that the IVD region can stabilize a homodimeric structure and that NEMO's KBD and IVD form a single structural unit that undergoes a cooperative unfolding transition, at least in the context of the truncated construct NEMO 44 to 195.The IVD is only seen to be destabilizing when the downstream HLX2 and CC2/LZ domains are also present, as, for example, in fulllength NEMO.Our findings imply that the IVD has an organized three-dimensional structure with an interface between the two chains of the NEMO homodimer that is not intrinsically destabilizing, but in the context of full-length NEMO the IVD becomes strained so as to destabilize the overall protein.This strain implies that the upstream KBD and the downstream HLX2/LZ domains place conflicting geometric or conformational demands on the IVD by the upstream KBD and the downstream HLX2/LZ domains.We propose that substituting the core region of the IVD with the highly flexible 9SG sequence relieves this strain by disrupting the structural integrity of the IVD such that it can now simultaneously conform to the geometric preferences imposed by the KBD on one side and by the HLX2/CC2/LZ domains on the other.In contrast, the L153R disease mutation causes the IVD to destabilize NEMO to an even greater extent than occurs in WT NEMO.Thus, either decreasing or increasing the destabilizing interactions of the IVD can interfere with NEMO function.Consistent with the 9SG and L153R mutations differentially affecting flanking regions of NEMO, the two mutations also have different effects on the interaction of NEMO with TRAF6: that is, 9SG only minimally affects the interaction with TRAF6, whereas the L153R mutation severely reduces the interaction (Fig. 5D).Of note, the regions of NEMO that interact with TRAF6 have been mapped to areas both N-and C-terminal to the IVD (22), again suggesting that the IVD mutations affect the structure of flanking domains.
Overall, it appears that the core IVD is crucial to balancing the conflicting conformational requirements of the flanking regions of NEMO, placing the two ends of the protein in allosteric communication with each other.We propose that the proper function of NEMO depends on a delicate communication between the strained IVD and the adjacent domains, and on how ligand binding to these flanking domains redistributes this strain across the different regions of the protein.
One explanation for how this strain is imparted to the IVD is that the IVD, like its neighboring domains, normally exists as an α-helical coiled-coil, but is capable of adopting two different intra-dimer interaction angles between the helices (Fig. 7).If one twist angle between the IVD helices were most compatible with the KBD dimer structure, but a different twist angle fit better with the constraints imposed by the downstream HLX2/CC2/LZ domains, then the intra-chain interactions within the IVD could be stabilizing in a KBD-IVD construct, as we observed experimentally, but would be destabilizing in the full-length protein as the IVD cannot simultaneously satisfy the conflicting geometric demands of the KBD and HLX2/CC2/LZ domains.One alternative to this "IVD helical twist" model is that in the KBD/IVD construct NEMO 44 to 195, only the upstream parts of the IVD form a stabilizing coiled-coil interaction whereas the downstream IVD segments are disordered or do not contact each other.In this scenario, when the HLX2/CC2/LZ domains are present they force an association between the downstream regions of the IVD that enforce energetically unfavorable contacts.
One key question is how the accumulation of strain energy in the IVD is utilized in NEMO's function.The use of binding energy derived from favorable interactions in opposition to destabilizing interactions elsewhere in the same protein is known to be a means by which proteins achieve and regulate their functions (46,47).We previously showed that binding of an IKKβ-derived peptide to the KBD of NEMO induces a conformational change in NEMO (13).In light of the mechanism of IVD function proposed herein, this previous finding can be interpreted as IKKβ peptide binding acting to lock the KBD into the rigid geometry that is observed experimentally (38), thereby modulating the geometric constraints on the nearby IVD.It is plausible that altering the geometry of these portions of the highly strained IVD causes a change in IVD conformation, in this case resulting in a "kink" in the protein, as seen in the IVD region by small angle X-ray scattering (13).
The key role that IVD strain plays in this conformational change is shown by the observation that introduction of the flexible 9SG mutation abolishes this conformational effect of IKKβ peptide binding (13) (Fig. 7).In cells, the association of NEMO with IKKβ is likely constitutive, based on the high affinity of this interaction (26), and therefore, the conformational change in NEMO that results from IKKβ binding is unlikely to reflect a process that is part of NEMO's signaling mechanism.However, it provides direct evidence for an intimate allosteric connection between the KBD and IVD, and for the importance of the IVD in determining the overall conformation of the NEMO dimer.Based on the allosteric communication that appears to exist between the KBD and the HLX2/LZ domains, we speculate that the conformational effects on NEMO seen upon binding of polyubiquitin to the UBAN site in the LZ domain (12,17) are also modulated by the IVD.
Our finding that the critical core region of the NEMO IVD can be substituted by the corresponding sequence from OPTN, with full retention of structure as measured by CD and disulfide-mediated dimerization assays and with full function as measured in cellular signaling assays, suggests that the IVDlike region of OPTN is both structurally and functionally analogous to the NEMO IVD.NEMO and OPTN have considerable overall aa sequence similarity and a similar domain organization, and in some cases OPTN can compete with NEMO for binding to ubiquitin (48,49), but their biological functions are largely distinct.That is, OPTN is a membrane-bound protein that has primarily been ascribed roles in autophagy, as a cargo receptor that transports ubiquitinated targets to lysosomes for degradation.Like NEMO, mutations in OPTN have been found in several human diseases, but OPTN-associated diseases are neurological diseases including ALS and glaucoma (50).Of note, two ALS mutations fall within the core region of the IVD-like region of OPTN that we used for our substitution experiments (50).Whether the IVD-like core sequence in OPTN also promotes LLPS is not known.It is of note, however, that OPTN may be part of pathogenic aggregates that form in ALS neurons (51).
One curious finding is that although single-residue human disease mutants have been found along the length of the NEMO protein ( 5), the 7XAla-NEMO mutant-which has seven Cys residues converted to Ala, spanning the region from aa 11 to 347-appears to be fully functional and structurally intact (31).The 7XAla-NEMO protein is useful for biochemical and biophysical analyses because, unlike WT NEMO, bacterially expressed 7XAla NEMO does not form disulfidebonded aggregates (31).We also found that 7XAla-NEMO forms a greater number of ubiquitin-induced LLPS droplets in vitro (Fig. 4B).One possibility is that 7XAla-NEMO (in the absence of disulfide bonds) can more readily transition to conformational states that promote LLPS upon binding to ubiquitin.Our comparative sequence analysis showed that the seven Cys residues are generally conserved in mammals (Fig. S4); however, they are not present in NEMO from many non-mammalian species (data not shown), which is consistent with our demonstration that these Cys residues are not essential for NEMO activity (31,32).
A limitation of our studies is that in our in vitro experiments we have examined the structure of NEMO in isolation or when bound to single ligands (e.g., ubiquitin or an IKKβ peptide).Clearly, in vivo NEMO, as a scaffold, binds to multiple protein substrates, including IKKβ, LUBAC, different poly-ubiquitins, TRAF proteins, IκBα, and perhaps others (21,34,(52)(53)(54).Furthermore, in cells, NEMO is subject to other contextdependent modifications, such as phosphorylation (55,56).All of these interactions and modifications are likely to affect the conformational states of NEMO.Nevertheless, because the conformational plasticity of NEMO is at the heart of dynamic events such as LLPS and signaling, understanding how different NEMO sequences contribute to conformational plasticity provides important fundamental scientific knowledge.
As we have recently reviewed (1), NEMO is one of several scaffold proteins that have been demonstrated to undergo context-dependent conformational changes that are important for directing and optimizing signaling events.Similarly, LLPS is important for many cellular and biomolecular events (57).Moreover, human disease mutations that affect dynamic structural changes in some other scaffolds have been documented (1), as we suggest herein for mutations within the IVD Figure 7. Models for role of the NEMO IVD in ligand-induced conformational change.Top Panel, in WT NEMO the IVD exists largely as an α-helical coiled coil and differing upstream and downstream constraints cause an asymmetric twist of angles between IVD helices that drive the helices towards diverging positions.In the presence of an activating signal for the NF-κB pathway, such as binding of TNFα to its receptor, the IKK complex binds to polyubiquitin chains localized to protein complexes at surface receptors.IKKβ binding to NEMO is proposed to lock the IVD into a strained geometry, causing an IVD-centered "kink" to act as a hinge for the observed conformational change in NEMO.Polyubiquitin binding to NEMO causing additional conformational effects may similarly be mediated by the IVD, already structurally primed from IKKβ binding, and leading to larger organizational changes of these protein complexes.This initiates the phosphorylation of IκBα, a signal for its K48 polyubiquitination and subsequent proteasomal degradation, freeing the NF-κB dimer to enter the nuclease, bind DNA, and upregulate its target genes.Bottom Panel, in 9SG NEMO, absent the directing of the native IVD core, ligand binding causes rigidification and a symmetrical tightening of the coiled-coils, of the upstream and downstream regions leading to an overall lengthening of the NEMO dimer.Without the intact IVD, NEMO is unable to undergo the ligand-induced conformational changes upon IKKβ and ubiquitin binding, and is unable to activate the NF-κB pathway.
of NEMO.Our results are consistent with a picture in which the IVD acts as a central hub that regulates the orientation of the flanking α-helical, coiled-coil domains that interact with key binding partners, and places these N-and C-terminal regions of the protein in allosteric communication with one another.Mutations in the IVD can destroy the structural integrity of this hub, and thereby impair the overall function of NEMO.Therefore, understanding how these conformational changes occur and how they are affected by disease could lead to the development of new types of therapeutics.Such strategies may be analogous to what has been done for the therapeutic stabilization of common mutations in the cystic fibrosis transmembrane conductance regulator (58).

Experimental procedures
Evolutionary rate, hydropathy, and similarity network analyses A BLAST database search was conducted for nonredundant protein sequences in the NCBI database using the human NEMO sequence as the query, yielding 500 sequences from vertebrate species with sequence identities ranging from 64 to 100%.The sequences were aligned using Clustal Omega (EMBL-EBI) (59) and formatted for input to the program Rate4Site (25) version 3.0.0,which was run using default settings to create a phylogeny tree, and then to calculate the evolutionary rate of each specific residue, with the output plotted and visualized using a four-period moving average.
The hydropathy of each individual residue was scored according to the Kyte & Doolittle hydropathy scale, plotted, and then subjected to a moving average for visualization.The evolutionary rates of ordered regions of NEMO with known structure were averaged, as were the evolutionary rates of uncharacterized regions of interest, which were divided by the ordered region average to obtain the indicated ratios.
For multiple sequence alignment (Fig. 1D), a BLAST database search using human NEMO as a reference sequence was conducted using Clustal Omega (EMBL-EBI).The alignment is visualized and colored according to residue conservation using MView (60).
For the SSN analysis (Fig. S3), an initial sequence BLAST search was conducted using human NEMO with a UniProt query e-value threshold of 1 × 10 −5 , with filtering to remove fragments and non-eukaryotic sequences using the EFI-EST tool.A final alignment score cut-off of 61% was used to obtain the set of sequences to conduct the SSN analysis with an e-value threshold of 1 × 10 −60 , and 674 sequences and 2708 nodes were imported into the program Cytoscape (61) for manipulation and visualization.

Plasmids
The pcDNA-FLAG expression vector has been described previously (11).cDNAs encoding WT and mutant IVDs were synthesized (GenScript or TWIST Bioscience) or generated via PCR (Table S1).The pcDNA-7XAla and 9SG variants have been described previously (13).These cDNAs and PCR-generated IVD mutants were subcloned into the appropriate vector using the cloning strategy outlined in Table S1.All plasmids were purified with a Midiprep kit (ZymoPURE, #D413) or a QIAprep spin miniprep kit (QIA-GEN, #27104) and verified by DNA sequencing prior to use.Primers used for PCR amplification of NEMO 252 to 419, 310 to 419, and 344 to 419 for the generation SUMO-tagged expression vectors are listed in Table S2.SUMO-NEMO-IVD clones were prepared in the pE-SUMOstar vector (Life Sensors PE-1106-0020) using the cloning strategies outlined in Table S1.
For NF-κB translocation and NEMO puncta formation analyses, DF-1 chicken fibroblasts were stimulated with the synthetic TLR2/6 antagonist Pam2CSK4 (InvivoGen tlrl-pms) at a final concentration of 10 μg/ml for the indicated times prior to lysis or fixation.Infection of DF-1 cells with mycoplasma was verified by the ATCC mycoplasma detection kit (ATCC 30-1012K) (Fig. S7).For analysis of phosphorylation of IκBα, 293T NEMO KO cells were stimulated with TNFα at a final concentration of 20 ng/ml for 10 min prior to lysis.To assess the formation of oxidation-induced NEMO dimers, cells were treated with 50 μM H 2 O 2 for 10 min prior to lysis.

Western blotting and co-immunoprecipitations
Whole-cell extracts from 293T cells were prepared as described previously (11,62) in AT lysis buffer (20 mM HEPES pH 7.9, 150 mM NaCl, 1 mM EDTA, 1 mM EGTA, 20% w/v glycerol, 1% v/v Triton X-100, 0.2% Protease Inhibitor Cocktail [Sigma-Aldrich P8340]).Samples analyzed under standard reducing conditions were heated at 95 C for 10 min in a loading buffer containing 5% β-mercaptoethanol prior to SDS-PAGE.Samples analyzed under non-reducing conditions did not contain β-mercaptoethanol and were either heated (Fig. 3B) or not heated (Fig. 3A) prior to SDS-PAGE.FLAG-NEMO and HA-TRAF6 co-immunoprecipitations were performed as described previously (32), and samples were separated by standard reducing SDS-PAGE.In all cases, extracts or co-immunoprecipitated proteins were separated on 10% SDS-polyacrylamide gels and then electrophoresed onto a nitrocellulose membrane in transfer buffer (20 mM Tris, 150 mM glycine, 20% methanol) overnight at 170 mA, 4 C.

Indirect immunofluorescence
Transfection and indirect immunofluorescence of DF-1 cells was performed essentially as described previously (33,62).Briefly, transfected DF-1 cells were seeded onto glass coverslips at an approximate density of 10 5 cells/coverslip and grown overnight.Cells were then washed twice with ice-cold PBS and then twice with saponin extraction buffer (80 mM Pipes, pH 6.8, 1 mM MgCl 2 , 1 mM EGTA, 0.1% saponin) for 10 min to select for higher order NEMO complexes (18,35).Cells were then fixed with 4% paraformaldehyde for 15 min at room temperature, blocked with blocking buffer (3% normal goat serum, 0.2% Triton-X-100 in PBS) for 1 h, and probed with an anti-FLAG primary antibody (1:100, #2368S, Cell Signaling Technology) diluted in blocking buffer for 2 h.Coverslips were washed three times with PBS for 5 min each and probed with an Alexa Fluor 488-conjugated goat-antirabbit secondary antibody diluted in blocking buffer (1:100, #A11008, Invitrogen) for 1 h at 37 C. Coverslips were then washed three times with PBS for 5 min each, counterstained with Hoechst (1:10,000, B2883-25MG, Sigma-Aldrich) for 1 min before mounting onto glass slides with SlowFade Gold antifade reagent (S36937, Invitrogen).Cells were examined under an Olympus IX70 inverted fluorescence microscope using an Olympus DPlanApo 40× UV objective.Images were analyzed in ImageJ utilizing Otsu thresholding and counting the number of puncta with a diameter of at least 1 μm.An F-test was first performed to determine the equality of variance, and then either a Student's t test, or a Welch's t test was performed to determine significance, based on the equality of variance.
All E. coli cells containing vectors for bacterial expression of NEMO proteins were plated on Luria broth (LB) agar plates containing the appropriate antibiotic and were incubated overnight at 37 C. A single colony was picked and used to inoculate 50 ml of LB containing the appropriate antibiotic(s) and incubated overnight at 37 C with shaking at 200 rpm.Five ml of the overnight culture was used to inoculate 1 L of LB containing the appropriate antibiotic(s) and incubated at 37 C with shaking at 250 rpm until the culture reached an OD 600 of 0.6.Isopropyl β-D-1-thiogalactopyranoside (IPTG) (Gold-Bio) was then added to a final concentration of 1 mM, and cells were grown for another 4 h.Cells were then isolated by centrifugation at 6000g for 15 min at 4 C, the supernatant was discarded, and the pelleted cells were stored at −80 C.
For purification, pelleted cells from 4 L of LB were thawed for 30 min at room temperature and solubilized in 60 ml of lysis buffer containing 20 mM sodium phosphate (Sigma-Aldrich) pH 7.5, 500 mM NaCl (RPI Research Products International), 40 mM imidazole (RPI Research Products International), two EDTA-free Pierce protease inhibitor tablets (Thermo Fisher), 600 μl of 10 mg/ml DNAse I (GoldBio) with all purification buffers for full-length WT NEMO also containing 5 mM Tris(2-carboxyethyl)phosphine (TCEP) (GoldBio) to minimize the formation of disulfide0bonded aggregates.Resuspended cells were lysed using a M110P microfluidizer (Microfluidics) at 18,000 psi and 8 M urea was added and incubated with stirring for 1 h at room temperature to dissociate contaminating proteins and prevent them from copurifying with NEMO.The cell debris was pelleted by ultracentrifugation at 35,000 rpm for 30 min at 4 C, and then the supernatant was sonicated to shear remaining DNA fragments.The remaining purification steps were carried out at 4 C using an ÄKTA protein purification system (GE Healthcare/Cytiva) for column chromatography steps.Specifically, the clarified lysate was added to a 5 ml HisTrap High Performance column (GE Healthcare/Cytiva), washed with 5 column volumes of lysis buffer supplanted with 6 M urea, with the bound protein refolded on-column using a urea gradient from 6 to 0 M urea over 20 column volumes.The protein was eluted using an imidazole gradient made with lysis buffer that was supplanted with 500 mM imidazole.The eluted protein was concentrated using Amicon Ultra-15 Centrifugal Filter Units (Millipore-Sigma) centrifuged at 4000g to no more than 3 ml, and then purified using size exclusion chromatography (SEC) to remove contaminating proteins, NEMO dimers, and other higher order oligomers that were present during purification of constructs containing significant portions of the coiled-coiled dimerization domains.The SEC was performed using a HiPrep 26/60 Sephacryl S-300 HR column (GE Healthcare/Cytiva) in storage buffer containing 20 mM sodium phosphate pH 7.5, 500 mM NaCl, with a flow rate of 0.6 ml/min, and collected in 3 ml fractions, Fractions containing monomeric NEMO were pooled, concentrated as above to 1 to 5 mg/ml, flash frozen in liquid nitrogen, and stored at −80 C. For NEMO constructs containing an Nterminal SUMO fusion protein, the SEC step was omitted.Instead, the SUMO tag was removed, by first concentrating the eluate from the HisTrap column as above to 1 ml, diluting 5× with storage buffer containing 1 mM DTT (GoldBio), and incubating with either 2.5 μl (25 units) of SUMOstar Protease 1 (LifeSensors) for constructs in SUMOstar vectors or with SUMO Protease (Thermo Fisher) for constructs in non-SUMOstar vector for 4 h at 25 C followed by incubation at 4 C overnight.The reaction mixtures were then loaded onto a 5 ml HisTrap High Performance column, and the flowthrough fractions containing the untagged NEMO proteins were collected.concentrated, and flash frozen as described above.The purifications of NEMO 252 to 419, NEMO 310 to 419, and NEMO 344 to 419 were conducted without the denaturation step, as contaminating proteins copurifying with other NEMO constructs were not visible during the purification of these constructs.
Protein concentrations for the constructs NEMO 1 to 419 WT, NEMO 1 to 419 7XAla, NEMO 1 to 419 9SG, NEMO 1 to 419 OPTN-IVD, NEMO 1 to 419 L153R, and NEMO 44 to 419 were calculated from absorbance at 280 using the calculated extinction coefficient of each construct, calculated with the ProtParam tool on the Expasy web server (63) and measured using a NanoDrop 2000 (Thermo Fisher).For NEMO 110 to 195, the concentration was calculated by means of the Lowry protein assay using Folin & Ciocalteu Reagent (Thermo Fisher).For all other proteins, the concentration was calculated by the Bradford assay using a Quick Start Bradford Protein Assay Kit 2 (Bio-Rad).

SDS-polyacrylamide gel electrophoresis of bacterially expressed proteins
Samples analyzed by SDS-PAGE were separated under standard reducing conditions and were boiled at 95 C in a loading buffer containing a final concentration of 5% β-mercaptoethanol.Proteins were then separated on 10 to 20% SDSpolyacrylamide gels and were visualized by staining with Coomassie blue (Bio-Rad).

Peptide synthesis
The peptide fragment corresponding to NEMO residues 141 to 155 was synthesized using by Fmoc solid phase fast-flow peptide synthesis (64) with an N-terminal acetyl GC group.The reactor was filled with 190 mg of H-Rink Amide-ChemMatrix resin (Biotage) and swelled with dichloromethane (DCM) (Thermo Fisher, certified ACS) to help remove air bubbles, sealed, connected to the HPLC pump set at 8.3 ml/min, and rinsed with N,N-Dimethylformamide (DMF) (Thermo Fisher certified ACS) for several min while upside down with agitation until there were no visible air bubbles.The flow was then halted and the reactor placed in a 70 C water bath and allowed to equilibrate to 70 C for 5 min, followed by a two-min flush with DMF.A 0.4 M solution of 2-(7-Aza-1H-benzotriazole-1-yl)-1,1,3,3-tetramethyluronium hexafluorophosphate (HATU) (Chem-Impex International) was prepared in DMF and 2.5 ml was used to dissolve 1 mmol of each Nα Fmoc-protected aa (Chem-Impex International).Immediately prior to use, the aa solutions were activated with 1.56 eq of N,N-diisopropylethylamine (DIPEA) (Sigma-Aldrich, Reagent Plus, ≥99%), excepting cysteine where only 1.1 eq of DIPEA was used.Each activated aa was injected using a syringe pump at 8.3 ml/min for 30 s, for the coupling step, followed by a 40 s wash with DMF, a 20 s deprotection step with 20% piperidine (Sigma-Aldrich, Reagent Plus, 99%) in DMF to remove the Fmoc groups, and lastly a 1-min wash step with DMF.This process was repeated for each aa addition, followed by injection of 10 eq of acetic anhydride plus 10 eq of DIPEA in DMF, injected using a syringe pump at 8.3 ml/min for 36 s to acylate the N terminus, and then a final 2-min wash with DMF.The resin, containing the acylated peptide, was dried inside the reactor by pumping air through it.Then, the resin was removed, placed in a fritted syringe (Torviq), rinsed five times with DCM and two times with methanol, and dried under vacuum for 30 min.

Peptide cleavage from resin
The dried resin was transferred to a 15 ml conical tube, and a 10 ml cocktail of 2.5% water, 2.5% triisopropylsilane (TIS) (Sigma-Aldrich), 2.5% ethane-1,2-dithiol (EDT) (TCI America), and 9.25% trifluoroacetic acid (TFA) (Sigma-Aldrich) was added, followed by incubation for 3 h at room temperature with agitation.The solution was added to a fritted syringe and rinsed with 15 ml TFA into a 50-ml conical tube.The flowthrough containing the peptide was dried under a N 2 (g) stream until approximately 1 ml remained, at which point 15 ml of diethyl ether (Sigma-Aldrich, anhydrous, ACS grade) previously chilled to 4 C was added, following by vigorous shaking, and the tube was cooled in N 2 (l) for 2 min.The tube was centrifuged at 6000g at 4 C for 15 min, the supernatant was discarded, and the tube was left to dry at room temperature for 4 h.

Peptide purification
The successful synthesis and cleavage of the peptide was confirmed on a test sample using liquid chromatography/mass spectrometry (LCMS).Liquid chromatography separation was performed with a gradient between mobile phase A of water with 0.1% formic acid (Thermo Fisher, 99.0+%, Optima LC/ MS Grade) and mobile phase B of acetonitrile (Thermo Fisher, HPLC grade) with 0.1% formic acid, using a 30 mm by 2.1 mm C18 column with 3 μm sized particles (Waters) using a Q-TOF Premier LCMS system (Waters).The dried peptide was then dissolved in 1.5 ml of 50:50 water:acetonitrile and purified using a 25 g C8 column (Interchim) on a puriFlash XS520Plus system (Interchim), with a mobile phase A of water with 0.1% TFA and a mobile phase B of acetonitrile with 0.1% TFA, and collected in fractions.The fractions were checked by LCMS to identify those containing pure peptide, and these fractions were pooled, dried using a centrifugal Genevac EZ-2 Personal Evaporator, and stored at −20 C. Peptide concentrations were determined by the Lowry protein assay using Folin & Ciocalteu Reagent (Thermo Fisher).

Protein labeling and in vitro liquid-liquid phase separation assay
Bacterially expressed and purified NEMO proteins were labeled with the Alexa Fluor 488 Microscale Protein Labeling Kit (Thermo Fisher, A30006) according to the manufacturer's instructions, using a molar ratio of 10:1 for Alexa Fluor 488:NEMO.The final degree of labeling of NEMO proteins was calculated to be approximately two molecules of Alexa Fluor 488 dye per molecule of NEMO.
To measure phase separation of NEMO proteins, 6 μM of labeled NEMO protein was incubated with concentrations from 0 to 2.25 μM of M1-Ub 8 (#BML-UW0805-0100, Enzo Life Sciences) in a buffer containing 20 mM Tris-HCl, pH 7.5, 150 mM NaCl, 5 mM ATP, 2 mM DTT, and 0.2 mg/ml BSA.Mixtures ((total volume 20 μl) were incubated at 37 C for 30 min in 384-well plates (that were previously coated for 1 h with 30 mg/ml BSA at 37 C) (#3544, Corning).Imaging was then performed on a Nikon C2+ confocal microscope equipped with a thermal chamber set to 37 C. Fluorescence intensities were measured by quantifying the total fluorescence using ImageJ.
Glutathione-S-transferase (GST) pull-down assays GST and GST-Ub 2 domain fusion proteins were expressed in BL21 E. coli cells and were purified from extracts using glutathione agarose (#16100, Thermo Fisher) as described previously (65).The GST proteins on glutathione beads were incubated with extracts from 293T NEMO KO cells expressing the indicated FLAG-tagged NEMO proteins (0.5% of each cell lysate was used as an input control).The beads were then washed four times with cold PBS, boiled at 95 C for 10 min in SDS sample buffer containing 5% β-mercaptoethanol, and electrophoresed on a standard denaturing 10% SDSpolyacrylamide gel.Pulled-down proteins were visualized by anti-FLAG Western blotting as described above (33, 62) (using anti-FLAG antiserum at 1:1000, #2368S, Cell Signaling Technology).To assess the amounts and sizes of the purified GST proteins used in the pulldowns, 1% of GST samples were electrophoresed on a 10% SDS-polyacrylamide gel, which was then stained with Coomassie brilliant blue (Bio-Rad).
Circular dichroism spectroscopy, and α-helical content and coiled-coil content determination The different purified NEMO proteins were diluted to 10 uM with 20 mM sodium phosphate buffer containing 500 mM NaCl at pH 7.4.CD spectra were obtained using an Applied Photophysics Chirascan CD spectrometer with a Starna Spectrosil quartz cuvette, 1 mm path length, under constant nitrogen flush.Buffer spectra were recorded from 190 to 260 nm with a 0.5 nm step at 0.5 s/step and at a temperature of 25 C.The raw signal, measured in millidegrees, was converted to mean residue ellipticity (deg cm 2 dmol −1 ), dividing by the number of residues (66).The ellipticity ratio used to evaluate the coiled-coil content of each construct was calculated from the ratio of the mean residue ellipticity measured at 222 nm to that at 208 nm.To determine alpha-helical content, each construct was concentrated to 335 μM, diluted to 10 μM with 97% with 2,2,2-trifluoroethanol (TFE), and the CD spectrum measured at 222 nm after incubation for 2 h at room temperature.This signal was compared to that of the construct similarly diluted and incubated, but in CD buffer rather than TFE, using Equation 1.

Thermal denaturation
Melting temperatures (T m ) were determined by monitoring the thermal denaturation of the proteins by CD.The raw signal at 222 nm was measured in millidegrees as the temperature was increased from 10 to 70 C at a rate of 1 C/min at a step of 0.2 C, with a tolerance of 0.15 C, a collection time of 0.5 s per time point, and was converted to mean residue molar ellipticity.The T m values were taken as the maximum of the first derivative of the thermal melting curves as a function of temperature.The reported T m values represent the average of at least 3 independent measurements, ± the standard deviation.

Urea denaturation
The purified NEMO proteins were concentrated to 100 μM and then diluted 10-fold with CD buffer supplemented with varying concentrations of urea.The samples were incubated at RT for 60 min to allow unfolding to occur.Incubation times of several hours were first tested to ensure that 60 min was sufficient time for equilibrium to occur.CD spectra of the samples were collected at 25 C from 190 to 260 nm in 0.5 nm steps, measured for 0.5 s/step, using a buffer containing the same urea concentration but without protein for background subtraction.The denaturation data were plotted after conversion from units of mdeg to MRE as above and fitted to the equation for two-state denaturation by nonlinear regression as previously described (40).The reported values for ΔG u represent the average of the results from at least two independent experiments.
To test whether the urea-induced denaturation was reversible, for each construct a 100 μM sample in CD buffer was diluted 1:1 with CD buffer containing 8 M urea and incubated for 60 min at RT.The samples were then diluted to 10 μM in CD buffer without urea followed by incubation for a further 60 min to allow refolding to occur.In each case, a control was conducted using CD buffer without urea for the denaturation step.CD spectra were measured as described above and compared to ensure the NEMO constructs were able to fully refold.

NanoDSF
Nano differential scanning fluorimetry data were collected using a NanoTemper Prometheus NT.48 instrument.NT.48 series nanoDSF grade standard capillaries (Nanotemper) were loaded with 25 μl of protein at 1 to 5 mg/ml in 20 mM sodium phosphate buffer, pH 7.4, 500 mM NaCl.The tryptophan and tyrosine residues were excited at 285 nm using 100% excitation power, and the resulting fluorescence was measured at 330 and 350 nm while the temperature of the sample was ramped from 20 to 70 C at a rate of 1 C/min.Using the included PR ThermControl software, the fluorescence data were plotted as a function of temperature and fitted to an arbitrary polynomial function.To determine the melting temperature, the first derivative of the ratio of the 350 to 330 nm curves was taken.

Figure 2 .
Figure 2. Effect of mutations in the core region of the IVD on the in vivo function and structural integrity of NEMO.A, IVD mutants were used in this study.Light blue residues indicate conservative amino acid substitutions, while orange residues indicate non-conservative substitutions.B, anti-NEMO (top) and anti-phospho-IκBα (bottom) Western blots of 293T NEMO KO cells transfected with the indicated FLAG-tagged NEMO expression vectors or an empty vector plasmid (−).Cells were treated with TNFα (final concentration 20 ng/ml) for 10 min prior to lysis (+) or left untreated (−) as indicated.Molecular

Figure 3 .
Figure 3. Mutations in a core region of the NEMO IVD affect its conformation and ability to form dimers. A, anti-NEMO Western blot of 293T NEMO KO cells transfected with FLAG-tagged expression vectors containing the indicated NEMO proteins or an empty vector (−).Samples were prepared in SDS sample buffer lacking β-mercaptoethanol and were not boiled prior to SDS-PAGE separation.NEMO, monomer, NEMO 2 , NEMO dimer.Molecular weight markers (in kDa) are indicated to the left.B, quantitation of the dimer/momomer ratio for three experiments performed as in (A).Shown are the means ± SEM; ns, not significant; *p < 0.05 by t test assuming equal or unequal variance as appropriate.Dots indicate values of individual experiments.C, anti-NEMO Western blot of 293T NEMO KO cells transfected as in (A).Cells were treated with 50 μM hydrogen peroxide for 10 min prior to lysis.Samples were boiled in SDS-sample buffer lacking β-mercaptoethanol prior to separation by SDS-PAGE.Molecular weight markers (in kDa) are indicated to the left.D, quantitation of the dimer/ monomer ratio for three experiments performed as in (C).Shown are the means ± SEM; ns, not significant; *p < 0.05 by t test assuming equal or unequal variance as appropriate.Dots indicate values of individual experiments.

Figure 4 .
Figure 4. Mutation of the core region of the NEMO IVD abolishes its ability to undergo liquid-liquid phase separation.A, fluorescent images of liquid droplets of the indicated NEMO proteins induced by M1-Ub 8 at different concentrations.Liquid droplets formed after mixing 6 μM of the indicated NEMO proteins with the indicated concentrations of M1-Ub 8 for 45 min at 37 C. Scale bar, 50 μm.B, quantitation of fluorescence intensity of the liquid droplets in (A).Shown are the means ± SEM, n = 10 areas, dots are the fluorescence intensities of individual areas.C, comparison of fluorescence at 1.1 μM Ub-8 for the indicated proteins.Values were normalized to the average intensity of WT NEMO (1.0).Shown are the means ± SEM, n = 10 areas, dots are the fluorescence intensities of individual areas.D, coomassie blue-stained gel of the bacterially expressed proteins used in (A).Samples were prepared in SDS sample buffer containing β-mercaptoethanol and were boiled prior to SDS-PAGE.E, Pull-down assay with GST and GST-Ub 2 of the indicated NEMO proteins.The top panel shows an anti-FLAG Western blot of GST or GST-Ub 2 pull-downs of lysates from 293T NEMO KO cells transfected with FLAG-tagged expression vectors containing the indicated NEMO proteins or an empty vector (−).Input lanes (I) contain 0.5% of the cell lysate used in each pulldown.The bottom panel is a Coomassie blue-stained gel of 1% of the GST and GST-Ub 2 proteins used in the pulldowns.For (D) and (E), molecular weight markers (in kDa) are indicated to the left.Dotted lines separate different sections of the same blot.
Dimerization has been observed by non-denaturing and native PAGE for constructs 44 to 419, 44 to 258, 44 to 195, 196 to 419, and 252 to 419 which include the flanking domains (data not shown).Inclusion of the adjacent KBD upstream of the IVD, in the construct NEMO 44 to 195 affords a fully cooperative thermal unfolding event, however, with T m = 44 C ± 1.4 deg.C, consistent with our prior work (13).A construct that includes the KBD and IVD plus the C-terminally adjacent HLX-2 domain (NEMO 44-258) also showed cooperative unfolding, having a T m = 36 C ± 1.0 deg.C, which is 4 C lower than full-length NEMO and 8 C lower than the KBD/ IVD construct NEMO 44 to 195 (Fig.

Figure 5 .
Figure 5. Inactivating mutations in the core region of the NEMO IVD affect puncta formation in vivo.A, anti-p65 Western blot of cytoplasmic (c) and nuclear (n) extracts from DF-1 cells treated for the indicated times with Pam2CSK at a final concentration of 10 μg/ml.Molecular weight markers (in kDa) are indicated to the left.B, representative fluorescent images of puncta formation in DF-1 cells transfected with the expression vectors for the indicated FLAGtagged NEMO proteins.Cells were treated with 10 μg/ml Pam2CSK for 4 h (+) or were left untreated (−) prior to saponin extraction and fixation.C, quantitation of puncta per cell of transfected cells in (B).Shown are the means ± SEM; n = ≥20 cells per condition in at least two experiments.ns, not significant; **p < 0.001 by t test assuming equal or unequal variance as appropriate.Dots are the number of puncta in individual cells.D, NEMO KO 293T cells were co-transfected with expression vectors for HA-TRAF6 and for the indicated FLAG-tagged NEMO proteins or an empty vector (−).Extracts from cells were immunoprecipitated with anti-FLAG beads (IP) and then subjected to Western blotting for either HA-TRAF6 or FLAG-NEMO.The lanes marked Input include 5% of the extract used in the given immunoprecipitations, also subjected to Western blotting.

Figure 6 .
Figure 6.Impact of the IVD on NEMO structure.A, protein domain map showing the residues present in each truncated NEMO construct.B, CDmonitored thermal denaturation of NEMO constructs measured by the loss in secondary structure monitored at 222 nm; inset showing the peak of the first derivative as the melting temperature (T m ).C, CD-monitored urea denaturation of NEMO constructs measuring the loss in secondary structure monitored at 222 nm (25 C and 10 μM), fitted to an equation for two-state denaturation by nonlinear regression.D, tabulated values of truncated NEMO constructs for the following: T m values determined via CD from (B) or via nanoDSF.The [urea] 50 indicates the concentration of urea at which half of the protein is unfolded from (C).The ΔG u values were calculated from linearized data in (C).The % helix was calculated from the CD spectra using the BeStSel server, and the ellipticity ratios were calculated from the signal at 222 nm to 208 nm.E, CD experiments conducted with 1 to 419 9SG, ± equimolar equivalents of a 45-mer peptide of IKKβ, incubated for 1 h at room temperature.The top panel shows the CD spectra (25 C; 10 μM) and the bottom panel shows the CD-monitored thermal denaturation with the inset showing T m as in (B).F, CD-monitored urea denaturation of NEMO measured as in (C).G, tabulated values for 1 to 419 7XAla NEMO and 1 to 419 9SG NEMO of the following: T m determined by CD in (E); the [urea] 50 in (F); the ΔG u values calculated from linearized data in (F); the % alpha-helix calculated by comparing the 222 nm protein signal in TFE to that in buffer; and the % helix and ellipticity ratios calculated as in (D).H, bar plots comparing the Δhelicity ratio and ΔT m for the indicated constructs ± IKKβ 45-mer peptide.